Rate of phagocytosis of Acanthamoeba species from groundwater. Part I.
Tasa de fagocitosis en las especies de Acanthamoeba provenientes de aguas subterráneas. Parte I.
Abstract
The Acanthamoeba species belonging to subclass of Gymnamoebia, a class of free living amoeba. Acanthamoeba species are cosmopolitan with distribution in the world. The phagocytosis is a biological mechanism more studied, especially in the pathogenic Acanthamoeba species. Many molecular mechanisms turn on the phagocytosis, between them carbohydrates recognition on the bacterial surface, and the activation of cellular mechanisms, as the phagosome formation and the fusion with the lysosomes for digestion. In the environment this function has been not studied, and here we have idea that this process is important in regulation of bacteria and others protozoa. The objective of work is the characterization of the phagocytosis of Acanthamoeba species isolated from Mezquital Valley groundwater (State of Hidalgo, Mexico). The phagocytosis was analyzed on non-nutritive agar Petri Plates with fluorescent label bacteria inoculate with Acanthamoeba and incubated by 30°C, 1 h, 1 ½ h, and then they were recovered to stained using to protocol of Sherr et al. (1993), modified. The phagocytosis of Acanthamoeba was higher to ½ hour of the interaction with fluorescent label bacteria (FLB), and reduced to 1 hour. The cellular process of phagocytosis showed in the work, started with thespecial pseudopods to trapped bacteria in floccules, forming phagosomes that regulated by time of the digestion.
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