Efecto de la administración de Limosilactobacillus reuteri vivo o muerto por calor en los niveles de algunos antioxidantes y citocinas en la suero de ratas expuestas al cadmio
Resumen
El propósito de este estudio fue examinar cómo la administración de Limosilactobacillus reuteri (L. reuteri), ya sea en su forma viva o muerta por calor, influye sobre los niveles de ciertos antioxidantes y citocinas en la sangre de ratas expuestas a la toxicidad crónica del cadmio. Para ello, se separó aleatoriamente a las ratas (n = 48) en seis grupos homogéneos : cadmio, L. reuteri vivo, cadmio + L. reuteri vivo, L. reuteri muerto, cadmio + L. reuteri muerto y grupo de control. Se administró cadmio por vía oral a los grupos cadmio, cadmio + L. reuteri vivo y cadmio + L. reuteri muerto tres veces por semana durante cuatro semanas. Se dio L. reuteri, ya sea vivo o muerto por calor, por vía oral a los grupos L. reuteri vivo, L. reuteri muerto, cadmio + L. reuteri vivo y cadmio + L. reuteri muerto durante el mismo tiempo. Tras el periodo de tratamiento, se obtuvieron muestras de suero de los animales. Se estudiaron los niveles de cadmio y glutatión peroxidasa en la sangre, así como las actividades de las enzimas catalasa y superóxido dismutasa. Se evaluaron también los niveles de malondialdehído (MDA) y los niveles de factor de necrosis tumoral alfa (TNF - α), interleucina - 6 (IL - 6), IL - 10 e IL - 1β. La administración de L. reuteri, ya sea vivo o muerto por calor, redujo la acumulación de cadmio en muestras de suero de los animales de los grupos L. reuteri vivo y L. reuteri muerto (especialmente) en comparación con el cadmio (P < 0,05). La administración de L. reuteri vivo redujo la actividad de la enzima superóxido dismutasa en suero, mientras que, el L. reuteri muerto disminuyó los niveles de glutatión peroxidasa en comparación con el grupo control (P < 0,05). En conclusión, las cepas vivas y muertas de L. reuteri tuvieron diversos efectos protectores sobre los niveles séricos de citocinas en ratas, pero sus efectos sobre los indicadores de estrés oxidativo se limitaron.
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