Molecular characterization of feline Mycoplasmosis in cats treated at a clinic in the suburbs of Guayaquil, Ecuador, 2024.

Keywords: Feline mycoplasmosis, Mycoplasma haemofelis, molecular diagnosis, PCR, domestic cats

Abstract

Feline mycoplasmosis has gained relevance in Veterinary Medicine due to its ability to severely affect domestic cats, compromising their hematological status and increasing the risk of severe anemia. Additionally, clinical signs such as lethargy, anorexia, and pale mucous membranes may be present, which are sometimes difficult to diagnose using traditional methods. This study was conducted in a veterinary clinic in Guayaquil, Ecuador, with the aim of characterizing feline mycoplasmosis through molecular techniques and evaluating the diagnostic effectiveness of blood smear compared to Polymerase Chain Reaction. Blood samples were collected from 20 cats with clinical signs compatible with mycoplasmosis, resulting in 8 (40%) PCR-positive cases and 12 (60%) PCR-negative cases. Moreover, the most prevalent species was Candidatus Mycoplasma haemominutum (35%), followed by Candidatus Mycoplasma turicensis (15%) and Candidatus Mycoplasma haemofelis (10%). The blood smear showed low sensitivity (25%) but high specificity (91.7%) compared to the molecular technique. Likewise, a higher proportion of positive  cases was observed in adult cats and those with low body weight, although the differences were not statistically significant. The results confirm the importance of molecular diagnostics in cats suspected of having mycoplasmosis. This research provides local evidence of Mycoplasma circulation in felines, highlighting the need to adopt accurate detection tools to support therapeutic decision-making and disease control.

Downloads

Download data is not yet available.

References

Pérez Macchi S, Villalba M, Sepúlveda García P, Villagra V, Maldonado E, Brítez L. Diagnostico y análisis filogenético de la infección natural por Mycoplasma spp en felinos domésticos. Compend. Cienc. Vet. [Internet]. 2023 [Consultado 20 Ene 2025]; 13(2):11–7. Disponible en: https://goo.su/UuzHYw

Caballero Méndez L, Franco Montoya LN, Mazo MM, Sepúlveda JC, Valencia E, Portilla T, Restrepo L. Comparación diagnóstica entre análisis citológico y molecular para la detección de Mycoplasma haemofelis en gatos residentes de la ciudad de Pereira, Risaralda, Colombia. Rev. Investig. Vet. Perú. [Internet]. 2022; 33(1):e20432. doi: https://doi.org/h9tk DOI: https://doi.org/10.15381/rivep.v33i1.20432

Zhang Y, Zhang Z, Lou Y, Yu Y. Prevalence of hemoplasmas and Bartonella species in client-owned cats in Beijing and Shanghai, China. J. Vet. Med. Sci. [Internet]. 2021; 83(5):793–797. doi: https://doi.org/p784 DOI: https://doi.org/10.1292/jvms.20-0681

Ameldev P, Tresamol PV. Molecular Detection and Therapeutic Management of Feline Mycoplasmosis. IOSR J. Agric. Vet. Sci. [Internet]. 2017; 10(2):83–86. doi: https://doi.org/p785 DOI: https://doi.org/10.9790/2380-1002018386

Mesquita JR, Oliveira AC, Neves F, Mendoza JR, Luz MF, Crespo I, dos Santos T, Santos-Silva S, Vilhena H, Barradas P. Hemotropic Mycoplasma and Bartonella Species Diversity in Free-Roaming Canine and Feline from Luanda, Angola. Pathogens. [Internet]. 2021; 10(6):735. doi: https://doi.org/gkhz3v DOI: https://doi.org/10.3390/pathogens10060735

Razgūnaitė M, Lipatova I, Paulauskas A, Snegiriovaitė J, Karvelienė B, Zamokas G, Laukuté M, Radzijevskaja J. Prevalence and Diversity of Haemotropic Mycoplasma Species in Cats and Their Ectoparasites (Fleas and Ticks). Vet. Sci. [Internet]. 2024; 11(2):81. doi: https://doi.org/p786 DOI: https://doi.org/10.3390/vetsci11020081

Lee HA, Hong S, Chung Y, Kim O. Molecular Detection of Mycoplasma felis Infection in a cat with respiratory symptoms. J. Vet. Clin. [Internet]. 2018; 35(6):273–275. doi: https://doi.org/p787 DOI: https://doi.org/10.17555/jvc.2018.12.35.6.273

Hoseinpoor E, Goudarztalejerdi A, Sazmand A. Molecular prevalence and phylogenetic analysis of hemotropic Mycoplasma species in cats in different regions of Iran. BMC Microbiol. [Internet]. 2024; 24(1):198. doi: https://doi.org/p788 DOI: https://doi.org/10.1186/s12866-024-03356-8

Ferraz A, Barwaldt ET, Ongaratto RF, Bierhals ES, de Lima CM, Dallmann PRJ, Moreira TFB, do Santos RL, Cunha RC, Schuch LFD, Nobre MO, Nizoli LQ. Molecular diagnosis of Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ in Domestic feline: a case report. Adv. Res. [Internet]. 2024; 25(4):372–378. doi: https://doi.org/p789 DOI: https://doi.org/10.9734/air/2024/v25i41115

Mayorga D, Echeverry DF, Buriticá-Gaviria E, Rondón- Barragán I. Mycoplasma haemominutum en la ciudad de Ibagué (Colombia): reporte de cinco casos. Revis. Investig. Vet. Perú. [Internet]. 2019; 30(3):1351–1359. doi: https://doi.org/p79b DOI: https://doi.org/10.15381/rivep.v30i3.15527

Hurrass C, Mora B, Gonzalez P, Tamiozzo P. Molecular detection of Mycoplasma spp. in cats with and without Feline Respiratory Disease Complex-compatible clinical signs. Ab Intus FAV-UNRC. [Internet]. 2024 [cited 22 January 2025];13(7):37–42. Disponible en: https://portal.amelica.org/ameli/journal/820/8205059004/

Cornejo Guerrero Emilia Catalina, Flores Medina Andrea Estefania, Guzmán Ramos Marlon Sebastián. Mycoplasmosis in domestic felines (Felis silvestris catus): Review and prevention proposals. CIENCIAMATRIA [Internet]. 2024 [cited 13 January 2025];10(2). Disponible en: https://www.cienciamatriarevista.org.ve/index.php/cm/article/view/1466

Valle-Mieles E, Villamarín-Barragán D, Mieles-Soriano G, Valle-Garay M. Complicaciones hematológicas causadas por la presencia de Mycoplasma spp. en gatos con leucemia viral felina. Rev. Cient. FCV-LUZ [Internet]. 2022; 32: 32165. doi: https://doi.org/p79f DOI: https://doi.org/10.52973/rcfcv-e32165

Lobová D, Konvalinová J, Bedáňová I, Filipejová Z, Molinková D. Infections of cats with blood mycoplasmas in various contexts. Acta Vet. Brno. [Internet]. 2021;90(2):211–219. doi: https://doi.org/p79h DOI: https://doi.org/10.2754/avb202190020211

Imre M, Văduva C, Dărăbuș G, Morariu S, Herman V, Plutzer J, Suici T, Lait P, Imre K. Molecular detection of hemotropic mycoplasmas (hemoplasmas) in domestic cats (Felis catus) in Romania. BMC Vet. Res. [Internet]. 2020;16(1):399. doi: https://doi.org/p79j DOI: https://doi.org/10.1186/s12917-020-02626-7

Ríos–Usuga C, Arias A, Gómez D, Pérez D, Muñoz–Cadavid C, Jaramillo–Delgado IL. Enfermedades transmitidas por vectores en gatos: una mirada molecular en ambientes urbanos de Medellín, Colombia. Rev. Med. Vet. Zoot. [Internet]. 2023; 70(2):220–233. doi: https://doi.org/p79m DOI: https://doi.org/10.15446/rfmvz.v70n2.105407

Malangmei L, Ajith Kumar KG, Nandini A, Bora CAF, Varghese A, Amrutha BM, Kubert P, Pradeep R, Nimisha M, Deepa C, John L, Ravindran R. Molecular Characterization of Hemoparasites and Hemoplasmas Infecting Domestic Cats of Southern India. Front. Vet. Sci. [Internet]. 2021; 7:597598. doi: https://doi.org/g5v9mc DOI: https://doi.org/10.3389/fvets.2020.597598

Published
2025-10-09
How to Cite
1.
Carrillo-Chuquitarco BD, Mendoza-Castañeda G, Zambrano-Guerra M del C, Torres-Lasso P, Cercado-Malavé LI, Macías-García CN. Molecular characterization of feline Mycoplasmosis in cats treated at a clinic in the suburbs of Guayaquil, Ecuador, 2024. Rev. Cient. FCV-LUZ [Internet]. 2025Oct.9 [cited 2025Nov.13];35(3):5. Available from: http://www.produccioncientifica.luz.edu.ve/index.php/cientifica/article/view/44623
Section
Veterinary Medicine