Demonstration of ovarian and brain tissue interactions as a result of stimulation of oogenesis in Migraine Modeled rats.
Abstract
Migraine, is a primary neurological disease. Although there is no gender difference in the prepubertal period, migraine is more common in adult women than in adult men. This situation decreases with menopause, but still its dominance in women continues. In our study, it is aimed to determine whether follitropin alfa could have a developmental benefit on the ovarian follicles of animals in migraine model after creating experimental migraine model with nitroglycerin on rats. In this study, the animals were divided into a total of four groups: a control group and three experimental groups. The first experimental group animals were injected with nitroglycerin, the other groups were injected with follitropin alfa and follitropin alfa together with nitroglycerin. At the end of 21 days, euthanasia was provided with pentothal sodium. Immunohistochemical methods were applied to post-mortem brain and ovarian tissues of rats. c-Fos was used as a migraine marker, Zona Pellucida 3 was used to show changes in the zona pellucida on the ovarian surface, MMP-9 and transient receptor potential vanilloid 1 were used to elucidate the pathogenesis of migraine. In our study, samples were evaluated by comparison with immunohistochemical staining. In animals, high c-Fos localization in the brain stem, high expression of Matrix metalloproteinase-9 in granular neurons, transient receptor potential vanilloid 1 in theca layer and Zona Pellucida 3 in the zona pellucida region were detected. The expression relationships of c-Fos, Matrix metalloproteinase-9, transient receptor potential vanilloid 1 and Zona Pellucida 3 in the brain stem, brain frontal cortex and ovary tissues where migraine and follitropin alfa were studied together have been shown for the first time in this study.
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