Caracterizacion fenotípica y genotípica de cepas aisladas de Flavobacterium psychrophilum en granjas de jaulas de trucha arcoíris en la ciudad de Kahramanmaraş, Turquía
Resumen
Esta investigación tuvo como objetivo caracterizar fenotípica y genotípicamente cepas de Flavobacterium psychrophilum en juveniles de trucha arcoíris (Oncorhynchus mykiss) de 68 granjas de jaulas ubicadas en Kahramanmaras, Turquía. El presente estudio se realizó entre diciembre de 2022 y julio de 2023, durante el cual se recolectaron especímenes de peces juveniles (con un peso de 5 g o menos) junto con individuos de mayor tamaño. El aislamiento de la bacteria se llevó a cabo mediante la utilización del medio Tryptone Yeast Salt Agar, el cual fue incubado a una temperatura de 15°C por un período comprendido entre 5 y 7 días. El procedimiento llevado a cabo consistió en la purificación de las colonias amarillas, para lo cual se empleó el sistema de microplacas GENIII de Biolog, con el propósito de realizar las pruebas bioquímicas necesarias para la evaluación de los rasgos fenotípicos. En el ámbito de la validación molecular, la identidad de las cepas identificadas inicialmente por Biolog fue confirmada mediante la técnica de Reacción en Cadena de la Polimerasa con cebadores específicos. Todos los aislamientos se sometieron a análisis de secuencia y los datos resultantes se compararon con las secuencias de referencia del NCBI. Se identificaron de manera concluyente doce cepas de F. psychrophilum mediante análisis fenotípico y genotípico combinado.
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Kayaci A, Büyükçapar HM. Kahramanmaraş İlinde Karada Su Ürünleri Yetiştiriciliği Yapan İşletmelerin Yapısal ve Biyoteknik Analizi [Structural and bio–technical analysis of aquaculture farms in land in Kahramanmaras]. KSÜ Doğa Bil. Derg. [Internet]. 2011 [cited May 22, 2025]; 14(4):1-6. Turkish. Available in: https://goo.su/IqJNUO
Ekman E. Natural and experimental infections with Flavobacterium psychrophilum in salmonid fish. [doctoral thesis on the Internet]. Uppsala (Sweden): Swedish University of Agricultural Sciences. 2003 [cited 13 Sep, 2022]; 47 p. Available in: https://goo.su/TJRFUpz
Cipriano RC, Holt RA. Flavobacterium psychrophilum, cause of bacterial cold–water disease and rainbow trout fry syndrome. Fish Disease Leaflet No. 86. United States Dept. of the Interior. U.S. Geological Service, National Fish Health Research Laboratory, Kearneysville, WV. 2005; 44p. Available in: https://goo.su/TPCskB
Kubilay A, Altun S, Didinen BI, Ekici S, Diler Ö. Isolation of Flavobacterium psychrophilum in Farmed Rainbow Trout (Oncorhynchus mykiss). Kafkas Univ. Vet. Fak. Derg. [Internet]. 2009; 15(5):709–715. doi: https://doi.org/p55w DOI: https://doi.org/10.9775/kvfd.2009.093-A
Özcan M, Sarıeyyüpoğlu M. Investigation of the antibacterial susceptibilities of Flavobacterium psychrophilum isolated in some trout farms of Elazıg city. Aqua. St. [Internet]. 2013; 2:11-19. doi: https://doi.org/p553
Özcan F, Arserim NB, Ketani A, Aydın MK, Sever NK, Özcan N, Çelik BA. Culture–and Polymerase Chain Reaction–based detection of Flavobacterium psychrophilum in naturally ınfected rainbow trout (Oncorhynchus mykiss walbaum, 1792) from trout farms. BSJ Agri. [Internet]. 2023; 6(6):655-659. doi: https://doi.org/p554 DOI: https://doi.org/10.47115/bsagriculture.1313836
Özcan M, Sarieyyupoglu M. Identification and investigation of phenotypic and genotypic characteristics of Flavobacterium psychrophilum in fry rainbow trouts (Oncorhynchus mykiss) in some trout. Int. J. Sci. [Internet]. 2014 [cited 13 Sep, 2024]; 4(3):24-34. Available in: https://goo.su/3sE9W
Austin B, Austin DA. Bacterial Fish Pathogens: Disease in farmed and wild fish. 4th ed. UK: Springer Dordrecht. 2007. https://doi.org/cmq788
Nematollahi A, Decostere A, Pasmans F, Haesebrouck F. Flavobacterium psychrophilum infections in salmonid fish. J. Fish Dis. [Internet]. 2003; 26(10):563–574. doi: https://doi.org/fn4k44 DOI: https://doi.org/10.1046/j.1365-2761.2003.00488.x
Li S, Chai J, Knupp C, Nicolas P, Wang D, Cao Y, Deng F, Chen F, Lu T, Loch TP. Phenotypic and genetic characterization of Flavobacterium psychrophilum recovered from diseased salmonids in China. Microbiol. Spectr. [Internet]. 2021; 9(2):e0033021. doi: https://doi.org/p557 DOI: https://doi.org/10.1128/Spectrum.00330-21
İspir Ü, Şeker E, Sağlam N, Dörücü M. Doğu Anadolu Bölgesinde Bazı Gökkuşağı Alabalığı (Oncorhynchus mykiss) İşletmelerinde Görülen Flavobacterium psychrophilum Enfeksiyonunun Araştırılması [A study of Flavobacterium psychrophilum infection in some cultured rainbow trout (Oncorhynchus mykiss) in Eastern Anatolia]. F. Ü. Fen ve Mühendislik Bilim. Derg. [Internet]. 2004 [cited Dec 18, 2024]; 16(4): 718–724. Turkish. Available in: https://goo.su/4rYHBt
Barnes ME, Brown ML. A review of Flavobacterium psychrophilum biology, clinical signs, and bacterial cold water disease prevention and treatment. Open Fish Sci. J. [Internet]. 2011; 4:40-48. doi: https://doi.org/fjtmh5 DOI: https://doi.org/10.2174/1874401X01104010040
Ahmed I, Ishtiyaq S, Sayed SF. An overview on understanding the major bacterial fish diseases in freshwater salmonids. Front. Aqua. [Internet]. 2025; 4:1515831. doi: https://doi.org/p558 DOI: https://doi.org/10.3389/faquc.2025.1515831
Avila BW, Huyvaert KP, Winkelman DL, Fetherman ER. Factors affecting post–challenge survival of Flavobacterium psychrophilum in susceptible rainbow trout from the literature. Pathogens [Internet]. 2022; 11(11):1318. doi: https://doi.org/p559 DOI: https://doi.org/10.3390/pathogens11111318
Park J, Roh H, Lee Y, Lee JY, Kang HY, Seong MJ, Kang Y, Heo YU, Kim BS, Park CI, Kim DH. Characterization and pathogenicity of Flavobacterium psychrophilum isolated from rainbow trout (Oncorhynchus mykiss) in Korea. Microorganisms [Internet]. 2023; 11(10):2546. doi: https://doi.org/p56b DOI: https://doi.org/10.3390/microorganisms11102546
Farooq I, Ahmad N, Porter C, Smith R, Scharf T, Cowley A, Jenkins A, Yates JD, Hill JT, Nielsen BL. Characterization of halotolerant Kushneria isolates that stimulate growth of alfalfa in saline conditions. PLoS One. [Internet]. 2025; 20(5):e0322979. doi: https://doi.org/p56c DOI: https://doi.org/10.1371/journal.pone.0322979
Goller CC, Sjogren C. Preparing biolog growth plates. BIT– Protocols [Internet]. Barkeley (USA): Springer Nature. 2025 [cited Nov 18, 2024]. Available in: https://goo.su/zCcD5KU
Hussain SM, Sharif A, Bashir F, Ali S, Javid A, Hussain AI, Ghafoor A, Alshehri MA, Naeem A, Naeem E, Amjad M. Polymerase Chain Reaction: A toolbox for molecular discovery. Mol. Biotechnol. [Internet]. 2025. doi: https://doi.org/p56f DOI: https://doi.org/10.1007/s12033-025-01390-z
Nunes Ramos J, Veloso da Costa L, Viana Vieira V, Lima Brandao ML. Challenges in the ıdentification of environmental bacterial ısolates from the pharmaceutical ındustry facility by 16S rRNA gene sequences. Preprints [Internet]. 2025; 5:33. doi: https://doi.org/p56g DOI: https://doi.org/10.3390/dna5030033
Suzuki K, Arai H, Kuge T, Katagiri T, İzumi S. Reliability of PCR methods fort he detection Flavobacterium psychrophilum. Fish Pathol. [Internet]. 2008; 43(3):124-127. doi: https://doi.org/dmvvzj DOI: https://doi.org/10.3147/jsfp.43.124
Yıldırım S. Özer S. The existence of Flavobacterium spp. at rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) hatcheries in Caglarca village, Mersin–Turkey. J. Fish. Sci. [Internet]. 2010; 4(1):112-122. doi: https://doi.org/cm37wz DOI: https://doi.org/10.3153/jfscom.2010010
Dalsgaard I, Madsen L. Bacterial pathogens in rainbow trout, Oncorhynchus mykiss (Walbaum), reared at Danish freshwater farms. J. Fish. Dis. [Internet]. 2000; 23(3):199-209. doi: https://doi.org/dwsx7q DOI: https://doi.org/10.1046/j.1365-2761.2000.00242.x
Didinen BI, Diler Ö, Ekici S, Altun S. Identification of Flavobacterium psychrophium isolates using API ZYM and determination of antimicrobial suscebtibilities with ATB VET. J. Süleyman Demirel Univ. Eğir. Fish. Fac. 2005; 1(2):64-70. Available in: https://goo.su/9E382yX
Elsayed EE, Eissa AE, Faisal M. Isolation of Flavobacterium psychrophilum from sea lamprey, Petromyzon marinus L., with skin lesions in Lake Ontario. J. Fish Dis. [Internet]. 2006; 29(10):629-632. doi: https://doi.org/d73jz8 DOI: https://doi.org/10.1111/j.1365-2761.2006.00756.x
