Growth, body composition and digestive enzyme activities of rainbow trout fed with dietary Beta-glucan containing mannan oligosaccharide
Abstract
This study investigated the impact of a yeast containing the prebiotic β-glucans, on the growth parameters and enzyme activities of rainbow trout (Oncorhynchus mykiss) at various dosage levels. The fish were randomly placed in plastic tanks (120 x 50 x 32 cm) in three trial groups and replicates, with an average weight of 73.37 ± 0.21 g (n = 90). Three different diets were prepared; 0 g.kg-¹ in group G-1 (control), 0.5 g.kg-¹ of the prebiotic (mannan oligosaccharides + β-glucan) in group G-2 and 1 g.kg-¹ ratio prebiotic (mannan oligosaccharides + β-glucan) were added to group G-3. The fish were fed these experimental feeds for 60 days. By the end of the experiment, the G-2 group had achieved the highest average live weight and weight gain, with values of 155.38 ± 0.19 g and 81.76 ± 0.16 g respectively. Feed conversion ratio, specific growth rate, protein efficacy ratio and survival rate showed the best values (1.13 ± 0.03, 1.24 ± 0.003, 1.91 ± 0.001, and 93.33 ± 3.33, respectively) were observed in the G-2 group (P < 0.05). Pepsin activity was also significantly higher in the G-2 group (163.94 ± 2.23 U/mg protein; P < 0.05). Similarly, the highest trypsin, amylase, and lipase activities were recorded in the G-2 group, with values of 1.09 ± 0.05, 5.31 ± 0.22, and 4.38 ± 0.11 U/mg protein, respectively (P < 0.05). No significant differences were detected among the groups with respect to muscle proximate composition (P > 0.05). Both β-glucan–supplemented yeast groups positively influenced the growth performance and digestive enzyme activities of trout. However, the group receiving 0.5 g.kg-¹ β-glucan supplementation exhibited significantly superior results compared to the other groups (P < 0.05).
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