The effect of varied exercise intensity on antioxidant function, aortic endothelial function, and serum lipids in rats with non-alcoholic fatty liver disease.

El efecto del ejercicio de intensidad variada sobre la función antioxidante, la función endotelial aórtica y los lípidos séricos en ratas con enfermedad del hígado graso no alcohólico.

DOI: https://doi.org/10.54817/IC.v63n4a01
Keywords: different exercise intensity, NAFLD, aortic endothelial cell function, antioxidant, lipid metabolism

Abstract

This study aimed to compare the effects of diet and exercise of different intensities on antioxidant function, aortic endothelial cell function and serum lipids in NAFLD (nonalcoholic fatty liver disease) rats. Fifty Sprague-Dawley (SD) rats (180-220g) were randomly divided into two experimental groups and fed either a standard rodent chow diet (CON; n=10) or a high-fat diet (HFD; n=40). After 16 weeks, the animals that received the HFD were randomly separated into a high-fat control group (HFC; n=10) or three exercise training groups: HFD and low-intensity exercise (LE; n=10), HFD and moderate-intensity exercise (ME; n=10), and HFD and incremental intensity exercise (IE; n=10). These experimental rats keep sedentary or trained for the next six weeks. A detection kit was used to detect nitric oxide synthase (NOs), nitric oxide (NO), malondialdehyde (MDA) and other markers of aortic oxidative stress. The expression levels of endothelial nitric oxide synthase (e-NOS) and endothelin-1 (ET-1) were detected by immunohistochemistry. TC, TG, and other lipid metabolism parameters were detected by an automatic analyzer. Exercise with different intensities could improve lipid metabolism, enhance antioxidant function, reduce MDA (P<0.01), increase NO (P<0.01), and improve the expression of e-NOS and ET-1 (P<0.01) protein levels in NAFLD rats. Decreased blood lipids were exhibited in all exercise groups. Notably, the moderate-intensity exercise demonstrated more effect on increasing glutathione (GSH) contents (P<0.01) and decreased the expression of ET-1 protein levels (P<0.01). The results showed that exercise at different intensities improved lipid metabolism and enhanced anti-oxidation function. Moderate exercise could improve the function of aortic endothelial cells.

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Author Biographies

Ling Ruan, Xi’an Shiyou University. Xi’an, Shaanxi, China.

Department of Physical Education. Xi’an Shiyou University. Xi’an, Shaanxi, 710065, China. Phone 008618992591267.

Guanghua Wang, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Department of Physical Education, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Zhen Qing Lv, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Department of Physical Education, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Shoubang Li, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Department of Physical Education, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Qin Liu, Ankang University, Ankang, Shaanxi, China.

College of Physical Education, Ankang University, Ankang, Shaanxi, China.

Yiling Ren, Xi’an Shiyou University, Xi’an, Shaanxi, China

Department of Physical Education, Xi’an Shiyou University, Xi’an, Shaanxi, China

Quancheng Zhang, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Department of Physical Education, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Xianli Lv, Ankang University, Ankang, Shaanxi, China.

College of Physical Education, Ankang University, Ankang, Shaanxi, China.

Rongping Wu, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Department of Physical Education, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Zhan Jin, Xi’an Shiyou University, Xi’an, Shaanxi, China.

Department of Physical Education, Xi’an Shiyou University, Xi’an, Shaanxi, China.

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Published
2022-11-10
How to Cite
Ruan, L., Wang, G., Qing Lv, Z., Li, S., Liu, Q., Ren, Y., Zhang, Q., Lv, X., Wu, R., & Jin, Z. (2022). The effect of varied exercise intensity on antioxidant function, aortic endothelial function, and serum lipids in rats with non-alcoholic fatty liver disease.: El efecto del ejercicio de intensidad variada sobre la función antioxidante, la función endotelial aórtica y los lípidos séricos en ratas con enfermedad del hígado graso no alcohólico. Investigación Clínica, 63(4), 327-343. https://doi.org/10.54817/IC.v63n4a01
Issue
Vol. 63 No. 4 (2022): Investigación Clínica
Section
Articles